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SDS-PAGE Gel Casting: Common Problems & Fixes

Having problems casting SDS-PAGE gels? Often there are three signs that indicate issues with acrylamide gel casting: bands not being parallel, samples leaking out of the well while loading wells, and bands not separating properly. Indicators of SDS-PAGE Issues: 0:35 - Bands Are Not Parallel 1:44 - Samples Leaking Out 2:30 - Bands Not Separating More Resources Troubleshooting Gel Casting/ Polymerization Issues in SDS-PAGE - https://goldbio.com/articles/article/... Protein Electrophoresis Using SDS-PAGE: A Detailed Overview- https://goldbio.com/articles/article/... Protein Ladders: Prestained Versus Unstained - https://goldbio.com/articles/article/... Products GoldBio Protein Gel Electrophoresis Products - https://goldbio.com/collection/protei... Bis-Acrylamide - https://goldbio.com/product/bis-acryl... BLUEstain™ 2 Protein ladder, 5-245 kDa - https://goldbio.com/product/915/blues... Tris (Tris Base) - https://goldbio.com/product/3414/tris Glycine, USP Grade - https://goldbio.com/product/3237/glyc... Written, Edited, and Narrated by Karen Pierce _________________________________________________________________________________________ Gold Biotechnology, Inc. - https://www.goldbio.com At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media! Linkedin:   / gold-biotechnology-inc-   Facebook:   / goldbiotechnology   Twitter:   / goldbio   Instagram:   / goldbiostl