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From Smearing to Smiling: SDS-PAGE Troubleshooting Tips

Are you having problems running your SDS-PAGE gels? Discover the answers you've been looking for in this in-depth video on troubleshooting SDS-PAGE gel running issues. We break down six common issues while running your protein sample in an SDS-PAGE gel, including protein sample migration, smearing, and improper separation. For each issue we will provide analytical insight into what might have gone wrong, and then offer troubleshooting solutions to solve that problem. Indications of Problems 0:16 - Smeared Bands 0:46 - Proteins Ran Off Gel 1:10 - Smiling Shape in Gel 1:38 - Bands on the Edges are Distorted 1:55 - Proteins not Separated or Resolved 2:30 - Proteins Migrating Out of Wells More Resources Troubleshooting SDS-PAGE Gel Running Issues - https://goldbio.com/articles/article/... Protein Electrophoresis Using SDS-PAGE: A Detailed Overview- https://goldbio.com/articles/article/... Protein Ladders: Prestained Versus Unstained - https://goldbio.com/articles/article/... Products GoldBio Protein Gel Electrophoresis Products - https://goldbio.com/collection/protei... Bromophenol Blue Free Acid, ACS Grade - https://goldbio.com/product/3722/brom... BLUEstain™ 2 Protein ladder, 5-245 kDa - https://goldbio.com/product/915/blues... Tris (Tris Base) - https://goldbio.com/product/3414/tris Glycine, USP Grade - https://goldbio.com/product/3237/glyc... Written, Edited, and Narrated by Karen Pierce _________________________________________________________________________________________ Gold Biotechnology, Inc. - https://www.goldbio.com At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media! Linkedin:   / gold-biotechnology-inc-   Facebook:   / goldbiotechnology   Twitter:   / goldbio   Instagram:   / goldbiostl