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What is PCR and qPCR? | PCR Animation 3 года назад


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What is PCR and qPCR? | PCR Animation

Discover molecular biology and the Polymerase Chain Reaction (PCR). What is PCR and qPCR? PCR takes DNA, nucleotides, primers, and Taq Polymerase to make unlimited DNA. This target DNA gets seen by running it on an agarose gel in PCR or detecting it with a camera in qPCR. With qPCR probes labelled with different colours can amplify many targets per tube. PCR and qPCR get used in disease diagnosis and medical research. An example is respiratory infections. In respiratory infections, qPCR tests can detect the viruses and bacteria present. Thus, many targets get detected in one sample using probes in qPCR. 🌏 https://www.clevalab.com 👉 You may also like: The Basic Principles of a Cell,    • Understanding Cell Functions in the H...   📖 This video is also a blog post with images and a PDF Summary visit: https://www.clevalab.com/post/pcrandqpcr Visit the ClevaLab Channel Page for more videos:    / @clevalab   ✉️ Sign up to the email list to be notified of new videos: https://www.clevalab.com/email-subscr... SOCIAL MEDIA LINKS Twitter:   / _clevalab   Instagram:   / _clevalab   Pinterest: https://www.pinterest.com.au/ClevaLab Tiktok:   / clevalab   Facebook:   / clevalab   CHAPTERS: 00:00 Intro | The discovery of PCR 00:18 What is a primer? 00:34 DNA amplification by PCR 01:33 Agorose Gel visualisation of amplified DNA 01:59 Discovery of Thermus aquaticus (Taq) Polymerase 02:25 The first Thermal Cycler 02:41 The first real-time PCR (qPCR) instrument | Probes 03:22 qPCR amplification plot basics 04:20 Cycle Threshold (Ct) value 05:06 Standard Curve for DNA quantitation 05:25 Multiplex PCR | More than one target in one tube 05:46 PCR and qPCR uses 05:54 Respiratory virus detection with qPCR 06:35 Ct value in Contact Tracing for COVID-19 06:50 Summary #PCR #qPCR #ClevaLab

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