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Sanger DNA Sequencing, From Then to Now.

This video explores the basics of Sanger sequencing and the fascinating history behind this groundbreaking technology. It explains the basics of DNA sequencing, including the components necessary for the reaction and the steps involved. Topics also include ddNTPs, cycle sequencing, fluorescent dyes and capillary electrophoresis. We'll also compare Sanger sequencing to other sequencing technologies, such as NGS, to give you a better understanding of how it all works. Throughout the video, we'll take a chronological approach to explore the history of Sanger sequencing, starting with its development in the late 1970s and following its evolution. By the end of the video, you'll deeply understand Sanger sequencing and its role in sequencing the human genome. So grab a cup of coffee and journey through the fascinating world of Sanger sequencing! Whether you're a student, researcher, or just curious about the science behind DNA sequencing, this video is for you! 🌏 https://www.clevalab.com 📖 This video is also a blog post with images, references and a PDF download; visit: https://www.clevalab.com/post/sanger-... 👉 You may also like: "Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing."    • Next Generation Sequencing - A Step-B...   Visit the ClevaLab Channel Page for more videos:    / @clevalab   ✉️ Sign up to the email list to be notified of new videos: https://www.clevalab.com/email-subscr... SOCIAL MEDIA LINKS Twitter:   / _clevalab   Instagram:   / _clevalab   Pinterest: https://www.pinterest.com.au/ClevaLab Tiktok:   / clevalab   Facebook:   / clevalab   CHAPTERS: 00:00 The 1977 Invention of Sanger Sequencing 00:23 The Basics of How DNA Is Copied 00:36 What are dNTPs & ddNTPs anyway? 01:19 How Does a ddNTP Stop DNA Copying? 01:54 The DNA Naming Convention 5' to 3' 02:43 Which Bases Go Together in Base Pairing? 03:05 Steps of the First Sanger Sequencing Method 06:11 The First DNA Sequencing Instrument, the AB370A. 07:20 The Launch of the Human Genome Project 07:46 How Does Cycle Sequencing Improve Things? 08:59 Automated DNA Separation by Capillary Electrophoresis 09:36 ABI PRISM 310, Modern Sanger Sequencing 10:49 Fluorescent ddNTPs and the Invention of BigDyes 11:33 The Instrument That Sequenced the Genome 13:09 Why Use Sanger Sequencing When There's NGS? #ClevaLab #sequencing #sangersequencing