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LDL Cholesterol Uptake Assay: Live Cell Imaging Analysis-Cell Health Monitoring l Protocol Preview 2 года назад


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LDL Cholesterol Uptake Assay: Live Cell Imaging Analysis-Cell Health Monitoring l Protocol Preview

Watch the Full Video at https://www.jove.com/v/58564/ldl-chol.... LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring - a 2 minute Preview of the Experimental Protocol Portia Ritter, Keyvan Yousefi, Juliana Ramirez, Derek M. Dykxhoorn, Armando J. Mendez, Lina A. Shehadeh University of Miami Leonard M. Miller School of Medicine, Interdisciplinary Stem Cell Institute; University of Miami Leonard M. Miller School of Medicine, Department of Medicine, Division of Cardiology; University of Miami Leonard M. Miller School of Medicine, Department of Molecular and Cellular Pharmacology; University of Miami Leonard M. Miller School of Medicine, Dr. John T. Macdonald Foundation Department of Human Genetics; University of Miami Leonard M. Miller School of Medicine, John P. Hussman Institute for Human Genomics; University of Miami Leonard M. Miller School of Medicine, Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute; University of Miami Leonard M. Miller School of Medicine, Vascular Biology Institute; University of Miami Leonard M. Miller School of Medicine, Peggy and Harold Katz Family Drug Discovery Center; This protocol provides an efficient approach to measuring LDL cholesterol uptake with real time influx rates using a live cell imaging system in various cell types. This technique provides a platform to screen the pharmacological activity of compounds affecting LDL influx while monitoring for cell morphology and hence potential cytotoxicity. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments  

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