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CRISPR-Cas9 GE to Generate Jurkat Reporter Models for HIV-1 Infection | Protocol Preview

Watch the Full Video at https://www.jove.com/v/58572/crispr-c.... CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites - a 2 minute Preview of the Experimental Protocol Julia K. Bialek, Thomas Walther, Joachim Hauber, Ulrike C. Lange Leibniz Institute for Experimental Virology, Heinrich Pette Institute; University Medical Center Hamburg-Eppendorf, Department of Anesthesiology; German Center for Infection Research (DZIF),; We present a genome engineering workflow for the generation of new in vitro models for HIV-1 infection that recapitulate proviral integration at selected genomic sites. Targeting of HIV-derived reporters is facilitated by CRISPR-Cas9-mediated, site-specific genome manipulation. Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments