Скачать с ютуб Making synthetic human insulin в хорошем качестве

Making synthetic human insulin

In this video we explore the traditional way that synthetic, recombinant human insulin is produced, by synthesising the A and B chains separately in separate strains of E. coli, and then combined in vitro to make insulin. We look at how ampicillin and tetracycline resistance genes, and blue-white screening with X-gal are used to identify recombinant colonies, and the role of β-galactosidase in promoting transcription and as a reporter gene. We also look at the reason for creating fusion proteins, as a precursor to the final product, and other questions that are frequently asked about the process.. Images of proteins are generated from https://www.rcsb.org/3d-view/1MHI and https://www.rcsb.org/3d-view/ngl/6tbj. All other images © Andrew Douch. [email protected]