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Metagenomics tutorial for 16s rna data analysis Support my work https://www.buymeacoffee.com/informat... https://www.paypal.com/paypalme/thein... / bigdataanalytics One-on-One coaching (Video Conferencing) https://calendly.com/bioinformaticscoach One-on-One coaching(Audio Call) https://clarity.fm/vincentappiah Get more bioinformatics tutorials on Patreon / bigdataanalytics Subscribe to my channels Bioinformatics: / @bioinformaticscoach Data Science: / @datasciencecoach Short Clips: / @bioinformaticscforbeginners Reach out [email protected] my work Materials for this tutorial Original tutorial: https://training.galaxyproject.org/tr... https://github.com/marbl/Krona/wiki/I... krona taxonomy issues:https://github.com/marbl/Krona/issues... github page:https://github.com/vappiah/Metagenomi... porechop page:https://github.com/rrwick/Porechop#qu... kraken manual : https://github.com/DerrickWood/kraken... krona page fastp:https://github.com/OpenGene/fastp#fil... other kraken2 tutorials: • Metagenomic Sequence Classification u... How to install anaconda Linux • Install , Configure and Run Anaconda ... MacOS • Install, Configure and Run Anaconda i... - GUI Installer • Install, Configure and Run Anaconda i... - Command line installer fastqc binaries: • Fastqc Linux Install and Usage (Comma... conda: kraken2: • How I install metagenomics tools (KRA... how to install multiqc how to install krona: https://github.com/marbl/Krona/wiki/I... Chapters __________________________________________________________________________________________ 00:00 Intro and PC Requirement 01:35 Install tools using anaconda 05:26 Activate the conda environment 05:37 Create a working directory 08:40 Update the krona taxonomy databases 07:23 Get the Download links for the example data 08:54 Write a bash script to download the files 10:20 Add exection right to the bash script 10:44 Run the bash script to dowload the files 11:26 Move the fastq files to another directory 12:13 Quality Control 12:30 Run fastqc 14:28 Aggregate fastqc reports using multiqc 15:23 Open the QC Reports 18:39 Adapter and chimera removal 26:20 Filter reads using fastp 34:27 Download the Silva database 38:16 Sequence classification using kraken2 43:05 Visualize kraken classification reports using krona 47:23 Open the krona chart 49:01 Explanation of the krona chart commands used conda install conda create conda activate fastp porechop kraken2 metagenomics tutorial metagenomics sequence classification microbiome bioinformatics #bioinformaticsforbeginners #bioinformatics #bioinformática #linuxforbeginners #linux wget galaxy krona How I Classify Metagenomic Sequence Reads using KRAKEN | Episode 2 #bioinformatics #bioinformática #bioinformaticsforbeginners #genome #genomics #genomesequencing #datascience #metagenomics #linuxforbeginners metagenomics, metagenome, 16s rna, bioinformatics #microbiology