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Microbiology: Trichrome Staining Procedure

This video shows how to make a permanent slide using the Wheatley Trichrome Stain. The laboratory uses trichrome stained slides to identify protozoa and helminth structures found in an infected patient's gastrointestinal tract. Protozoa, which are often missed on the fecal concentration wet mount, are more visible due to the stain. Unlike wet mounts, the trichrome stained slide can be observed under oil immersion.