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Watch the Full Video at https://www.jove.com/v/57170/ecosyste.... Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions - a 2 minute Preview of the Experimental Protocol Jian Gao, Joelle Sasse, Kyle M. Lewald, Kateryna Zhalnina, Lloyd T. Cornmesser, Todd A. Duncombe, Yasuo Yoshikuni, John P. Vogel, Mary K. Firestone, Trent R. Northen Lawrence Berkeley National Laboratory, Environmental Genomics and Systems Biology Division; Department of Energy, Joint Genome Institute; Joint BioEnergy Institute,; University of California, Department of Environmental Science Policy and Management; This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory conditions. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments