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Lab 6 Overview: Purifying the Fluorescent Protein

This video provides a detailed overview of ABE's Lab 6: Purifying the Fluorescent Protein. In the first part of this laboratory, students use a reagent called “lysis buffer” to lyse (break open) the cells. In the second part of this laboratory, students use column chromatography to separate out the red fluorescent protein from the sample. The properties of the protein’s amino acids allow students to separate it from the rest of the sample using column chromatography. When students pass the sample through the column, the hydrophobic red fluorescent protein will stick to the resin beads, while the hydrophilic amino acids will pass through. Students will then wash out the remaining somewhat-hydrophobic proteins in the sample using a salt buffer solution. Finally, students will use another buffer solution to release the bound red fluorescent protein from the column and collect the product. This is the same process that would be used to isolate a human therapeutic protein. The Amgen Biotech Experience (ABE) is an innovative science education program that empowers teachers to bring biotechnology to their classrooms. ABE provides teacher professional development, curriculum materials, and research-grade equipment and supplies to secondary schools. Learn more at https://www.amgenbiotechexperience.com.