Скачать с ютуб Accelerate Your AAV Process Development With a New Wave Analytical Tool в хорошем качестве

Accelerate Your AAV Process Development With a New Wave Analytical Tool

Speaker: Andreja Gramc Livk , Sartorius BIA Separations Title of the lecture: Accelerate Your AAV Process Development With a New Wave Analytical Tool Date & Time: July 26, 2023 Adeno-associated viruses (AAVs) are widely used vectors for gene therapies mainly due to their favorable safety profile and high transduction efficiency to various target tissues. With increasing demand for faster process development of advanced therapies using AAVs, rapid at-line analytical chromatographic solutions are becoming indispensable. PATfix analytical system with multiple detectors seamlessly delivers coherent process information, considerably speeding up process development and enabling a fast-paced delivery of the product to the market. Analytical methods, embedded in the PATfix system that enables at-line monitoring of common product and process related impurities, provide a critical platform for the rapid development and characterization of AAV-based gene therapy vectors. OUR AAV LIBRARY: https://www.biaseparations.com/en/sea... GET in TOUCH: Webpage: https://www.biaseparations.com/ E-mail: [email protected] Tech support: [email protected] 0:00 Accelerate your AAV process development with a new-wave analytical tool 3:56 PATfix-Chromatographic Platform 5:26 PATfix Platform Applications 6:31 AAV Purification & Analytics Challenges 9:14 Impurities in AAV Production Process 10:27 AAV Purification Workflow 14:54 New Approach Needed for AAV Capsids Separation 16:15 Standard Analytical Methods for Analysis of Partially Filled AAV Capsids 17:30 Novel Method for High Resolution of AAV8 Capsids 20:46 Preparative Scale of Novel AAV Empty/full Method 21:34 PATfix Analysis of Preparative Fractions 30:08 Testing robustness: Ambr250 process replicates 33:13 PATfix® software-key analytical tool 35:45 Take home messages