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Metagenome-assembled Genome Extraction and Analysis

This webinar demonstrates how to extract metagenome-assembled genomes (MAGs) from your microbial community sequencing data. KBase scientist Dylan Chivian demonstrates the workflow from his recently published protocol for extracting MAGs from microbiome data and guide users through some of the analysis tools available on the platform. This process allows scientists to generate putative genomes from organisms found in the environment and understand their functional capacity and their role in the ecosystem. 0:00 Introduction to the webinar and protocol 4:35 Import and read quality 8:14 Read taxonomy - Kaiju 13:23 Metagenome assembly 17:18 Comparing assembled contigs 20:05 Contig binning 22:17 Optimizing binned contigs 25:05 Bin quality assessment 34:33 Extract individual assemblies 35:38 Annotate genomes 36:40 Taxonomic classification of MAGs with GTDBtk 39:30 Finding relatives with Species Tree 42:58 Placing genomes into phylogenetics context with phylum exemplars 44:40 Functional classification of MAGs 46:25 HMMER profiling and gene set extraction 48:00 Summarize GenomeSet Read and cite the article: https://doi.org/10.1038/s41596-022-00... Read and cite the Narrative: https://doi.org/10.25982/33233.606/18... Q&A Doc available here: https://docs.google.com/document/d/1F...