Скачать с ютуб How to make malt extract agar (MEA) for home mushroom cultivation в хорошем качестве

How to make malt extract agar (MEA) for home mushroom cultivation

Agar is quite simple when dealing with non-selective media. Standard 2% gelling and nutrient strength are all that are required to produce healthy fungal colonies. Amending with peptones, yeasts, etc, is not necessary but if that's your thing, to each their own; for most of us this will be all you'll ever need: for a sleeve of 25 @ 2% concentration: 600ml distilled h20 12g agar 12g Dry malt extract for a sleeve of 20 @ 2% concentration: 400ml distilled h20 8g agar 8g Dry malt extract Sterilization times are bioburden and volume-dependent. The larger the volume, the longer the exposure, the "dirtier" the liquid, the more time required to sterilize. 45 minutes for 1L and under will ensure that all solids have been uniformly dissolved into the water, and is a cheap insurance policy against potential failure.