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ABTS [TEAC] - Trolox-Equivalent Antioxidant Capacity Assay - Principle, Advantages & Limitations

This video explains about ABTS [TEAC] - Trolox-Equivalent Antioxidant Capacity Assay - Principle, Advantages & Limitations. Miller and Rice-Evans were the first to report the ABTS•+ assay in 1993, The assay is also known as TEAC [Trolox-Equivalent Antioxidant Capacity]. Initial Method: The generation of a radical cation (ABTS•+) was done by reacting metmyoglobin (Met-Myb) with hydrogen peroxide (H2O2) to produce hydroxyl radical (HO•). Improved Method: ABTS•+ radical generated by treating with Ammonium or Potassium persulfate in the ratio 1:0.5. Oxidation of ABTS reaction is long process and requires 12-16hrs of incubation. Principle: The TEAC Test (Trolox-Equivalent Antioxidant Capacity) is based on the reaction with the colored and relatively persistent 2,2 ′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+ ) radical cation which has a strong absorption band at 734 nm. DPPH Assay: Principle, Advantages & Limitations    • DPPH Assay: Radical Scavenging Activi...   Related Videos: --------------------------------------------------------------------------------------------------------------- Plotting Bacterial Growth Curve:    • Plotting Bacterial Growth Curve in Excel   Colony Forming Units:    • CFU: Colony Forming Unit & cfu/ml cal...   Serial Dilution Methods:    • Serial Dilution Methods & Calaculations   Copy Number Calculation for qPCR:    • Copy Number Calculation for qPCR   How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR:    • How to analyze Primer Sequence or Oli...   How to generate qPCR standard curve in excel and calculate PCR efficiency:    • How to generate qPCR standard curve i...   Real Time qPCR optimization, Calculating PCR Efficiency:    • Real Time qPCR  optimization, Calcula...   Taqman Assay Vs SYBR Green Assay:    • Real Time PCR Assays- Taqman Assay Vs...   Resolving poor PCR efficiency:    • Resolving Poor PCR assay Efficiency i...   Calculating molecular weight of Unknown protein    • How to calculate molecular weight of ...   Designing qPCR Primers    • Primer designing for real time PCR us...   Oligonucleotide Preparation & Purification    • Oligonucleotide Preparation and Purif...   How to check Oligo Concentration    • How to check the Oligo concentration ...