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E Seminar qPCR Assay Validation

Following the previous LGC e-seminars on quantitative PCR assay design and PCR assay optimisation, this e-seminar, entitled “An introduction to quantitative PCR assay validation”, will introduce the viewer to the topic of qPCR assay validation and provide best practice guidance on how to undertake the process. The information presented will equip viewers with the necessary knowledge and skills to ensure that methods are validated and fit for purpose. Key stages in the validation process are indicated, routinely employed evaluation parameters described and critical performance criteria highlighted. Links to useful resources, additional guidance and references are also provided. Those who should consider viewing this e-seminar include individuals currently working within the foods molecular testing area, particularly representatives from UK Official Control Laboratories, industry and members of organisations associated with the UK official control network. The production of this e-seminar was funded by Defra, FSA, FSS and BEIS under the Joint Knowledge Transfer Framework for Food Standards and Food Safety Analysis.