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Watch also Using wood ash as a sterilizer & substrate for macro propagation of plantain/ Banana(tissue culture) • Using wood ash as a sterilizer & subs... If you have any Job/partnership opportunity for me : Email: [email protected] / WhatsApp: +233249021373 ...Plantain sucker multiplication/Macro propagation Introduction... Tissue culture is the artificial multiplication of plant tissue or cells outside the parent plant. Main Aim/objectives -To replicate one sucker into 50-64suckers(peepers) -To produce a clean and healthy sucker free from nematodes within 2 to 4 months. Materials Needed *plantain sucker *saw dust *water source *watering can *nematicide *cutlass *knife *Rake *Hand gloves Procedure/Method 1.Remove a maiden sucker from the parent plant 2.Remove the outer layer using a knife. 3.cut the sword 4.Cross-cut the base containing the apical meristem into 4 equal parts ÷ each portion into two making 8 portions. 5.wash the 8cuttings in a solution of nematicide to get rid of nematodes 6.Make a bed and spread sawdust on it & sow the cuttings 7. Cover them with sawdust to facilitate germination & water depending on the soil moisture conditions. 8.sprouting of peepers(small suckers emerging from the soil) starts after 21 days. 9.Prick/remove the peepers & transplant them at the post nursery where they continue to grow for four months before they are transplanted on the main field. Note After 21 days , each cut replicates 8 peepers. which means , 8*8=*64suckers*can be obtained from just one sucker through tissue culture.