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Tumor Antigen-specific T Cells Generation for hiPSCs | Protocol Preview

Watch the Full Video at https://www.jove.com/v/59997/using-hu.... Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells - a 2 minute Preview of the Experimental Protocol Meghan L. Good, Raul Vizcardo, Takuya Maeda, Naritaka Tamaoki, Parisa Malekzadeh, Hiroshi Kawamoto, Nicholas P. Restifo National Cancer Institute, NIH, Surgery Branch; National Cancer Institute, NIH, Center for Cell-Based Therapy; Kyoto University, Laboratory of Immunology, Institute for Frontier Life and Medical Sciences; This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ+ single positive T cells using OP9/DLL1 co-culture system. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments