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Target Enrichment for Next-Generation Sequencing Analyses of SNPs, CNVs, Gene Fusions, and More

Targeted resequencing of both DNA and RNA allows researchers to focus on genes of interest to cost-effectively interrogate genetic variations in their samples such as SNVs, CNVs, and gene fusions. During this webinar we will describe and present data from a novel and flexible NGS target enrichment method that utilizes Single Primer Enrichment Technology (SPET). The approach can be used for a range of NGS-based analyses including identification of gene fusion events in RNA samples, targeted gene expression analysis, variant detection, and CNV analysis in DNA samples. The simple one-day protocol can generate sequence-ready libraries with as little as 10 ng DNA or total RNA from a broad range of samples. The enrichment assay is fully customizable to the user’s requirements. We will also discuss data generated with a 344 cancer gene panel using gDNA from both fresh and FFPE samples and a gene fusion panel designed to 500 genes implicated in fusion events (as defined in the Wellcome Trust COSMIC database, ChimerDB 2.0 and TCGA) with RNA from both primary and cultured cell lines.