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Let's explore why cloning vectors need to have few cloning sites for every restriction enzyme, but multiple cloning sites for multiple enzymes. We will also explore why the same restriction enzymes need to be used for the gene of interest and the cloning vector! Khan Academy is a nonprofit organization with the mission of providing a free, world-class education for anyone, anywhere. We offer quizzes, questions, instructional videos, and articles on a range of academic subjects, including math, biology, chemistry, physics, history, economics, finance, grammar, preschool learning, and more. We provide teachers with tools and data so they can help their students develop the skills, habits, and mindsets for success in school and beyond. Khan Academy has been translated into dozens of languages, and 15 million people around the globe learn on Khan Academy every month. As a 501(c)(3) nonprofit organization, we would love your help! Donate here: https://www.khanacademy.org/donate?ut... Created by Khadijatul Qubra