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Intermediate Fiji Workshop

This workshop is designed for users who want to process and quantify images using the open source program ImageJ/FIJI (Fiji.sc). It covers important topics for filtering and processing data, as well as particle detection, automatic selection generation and basic image calculations. Topics Covered -Processing images for quantification (Filters, LUTS, Background Subtraction, math) -Thresholding (monochrome & colour thresholding) -Analysing Particles -Automatically detecting objects and storing them into ROIs -Finding Maxima -Image Calculations (for Masking & FRET) Example files worked through here can be accessed from the following link: https://drive.google.com/drive/folder... This video was presented by Nicholas Condon at the ACRF Cancer Biology Imaging Facility at the Institute for Molecular Bioscience at The University of Queensland, Brisbane, Australia. For information about the IMB Microscopy facility visit: https://imb.uq.edu.au/microscopy