Скачать с ютуб Single-Particle Imaging to Quantitate Biophysical Properties of mRNA LNPs в хорошем качестве

Single-Particle Imaging to Quantitate Biophysical Properties of mRNA LNPs

In this NMIN lecture, Dr. Sabrina Leslie discusses a quantitative single-particle imaging platform that enables simultaneous measurements of the size, mRNA-payload, and dynamic properties of vaccines in cell-like conditions. Dr. Leslie’s team investigates the dependence of mRNA-lipid-nanoparticle structure and fusion dynamics on formulation, using commercially available formulations as a starting point. These measurements are made on confined, freely diffusing particles and during reagent-exchange, such as in response to solution pH, to emulate intracellular dynamics in a controlled setting. Dr. Sabrina Leslie is working at the interface of physics and biology with a particular interest in quantifying the dynamics of individual molecules. Dr. Leslie’s research provides insights into the biophysical properties of molecules and cells, including therapeutics with the ultimate goal of propelling quantitative health sciences.