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Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

References/Resources:   / the_crux   This video discusses the general biology of lambda bacteriophage vector and cosmid cloning vectors. There are two types - insertional and replacement - of vectors derived from lambda bacteriophage. This requires the use of spi phenotype since lambda phage vectors have no selection or screening markers. From the lambda bacteriophage vector, cosmids are created, relying on the unique function of cos sites. Understanding cosmid means understanding the structure and function of cos sites - a short section of this video explains the anatomy and function of cos sites in lambda phage and how it is used in lambda phage vector. Cosmid containing lambda phage viruses are assembled in vitro by extracting lambda phage proteins from bacteria and mixing it with the cosmid DNA in a tube. Lambda phage vectors and cosmids have specific complications when it comes to their application - most significant is the problem of cosmid concatermization and we discuss it towards the end of this video on lambda phage vectors. Jump to your favorite section: 00:00 Lambda Bacteriophage vector 02:12 Insertional vector 03:02 Replacement vector 04:30 spi phenotype 06:27 Cos site 08:27 Cosmids 10:58 In vitro phage packaging 12:14 Cosmid Concatemers 14:38 Phosmid Patreon Page:   / the_crux   Suggested videos: (PhiX174) Rolling Circle Amplification:    • Rolling Circle DNA Replication and Am...   OriC DNA replication:    • DNA Replication (Prokaryote) INITIATI...   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)