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Difference between RPKM/FPKM and TPM | RNA-Seq Normalization Methods | Bioinformatics 101 2 года назад


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Difference between RPKM/FPKM and TPM | RNA-Seq Normalization Methods | Bioinformatics 101

In this video, I talked about different RNA-Seq normalization methods - RPKM/FPKM and TPM and demonstrated how to calculate these values from counts. Leave your thoughts in the comment section below! Link to some reading material: 1. https://haroldpimentel.wordpress.com/... 2. https://www.rna-seqblog.com/rpkm-fpkm... Chapters 0:00 Intro 0:42 Types of Replicates 3:07 Biases (gene length and sequencing depth) 5:12 RPKM/FPKM and TPM normalization 7:16 Calculating RPKM values 11:26 Calculating TPM values 14:04 RPKM/FPKM vs TPM Show your support and encouragement by buying me a coffee: https://www.buymeacoffee.com/bioinfor... To get in touch: Website: https://bioinformagician.org/ Github: https://github.com/kpatel427 Email: [email protected] #bioinformagician #bioinformatics #rnaseq #fpkm #rpkm #tpm #normalization #rna #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #ngs

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