Скачать с ютуб Downstream processing in the pharmaceutical industry (Part I): recovery and purification в хорошем качестве

Downstream processing in the pharmaceutical industry (Part I): recovery and purification

Biopharmaceutical downstream processing refers to the recovery and purification of a molecule of interest from the host cells (for instance engineered E.coli). Typically, this is defined as the unit operations following the completion of cell growth in a bioreactor and subsequent harvesting. The purpose of the downstream processing is to isolate, purify and concentrate the molecule of interest from a complicated bulk matrix. Moreover, it can also involve packaging, distribution, and quality control strategies to optimise the process and scale up. In this video we will discuss some processes that are used in recovery and purification. Follow up videos will focus on practical examples and go more into details on how to concentrate, package the products, and how to perform quality control. 00:00 Intro 00:42 Downstream vs upstream 02:11 The basics of recovery 03:28 Cell disruption methods 04:45 Purification 06:11 Chromatography 07:50 Pressure swing adsorption 11:12 Role of sensors in the process 13:45 What's the next step?