Скачать с ютуб Total Flavonoid Content (TFC) Calculation in Microsoft Excel | Step-by-Step Beginner Tutorial в хорошем качестве

Total Flavonoid Content (TFC) Calculation in Microsoft Excel | Step-by-Step Beginner Tutorial

How to calculate total flavonoid content: 1) Plot the standard calibration curve: - plot absorbance vs standard concentration (10-100 ug/mL) - obtain the equation y=mx+c - ensure R2 is more than 0.95 y=absorbance, x=concentration, m=slope, c=intercept 2) Calculate the concentration of flavonoid content in samples using the equation y=mx+c - rearrange equation; x=(y-c)/m - x is the concentration of flavonoid content in ug/mL - convert ug/mL to mg/mL if necessary 3) Calculate the total flavonoid content (TFC) - TFC = cv/m c=concentration of flavonoid content v=volume of methanolic extract solution used m=weight of extract per mL in gram unit