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DESeq2 Design Explained: Understanding Interaction Terms in RNA-Seq Analysis

In this video, I'll break down the concept of interaction terms in #DESeq2 design, making complex statistical models accessible and easy to understand. In this tutorial I will guide you through - what are main effects vs interaction terms and why they are crucial in your experimental design, step-by-step examples of setting up and interpreting interaction terms in DESeq2, common pitfalls and best practices to ensure accurate and meaningful results. I hope you liked the video. I look forward to your comments under the comments section! ▸ Link to previous DESeq2 videos: 1.    • DESeq2 Basics Explained | Differentia...   2.    • DESeq2 workflow tutorial | Differenti...   ▸ Link to Vignette: https://www.bioconductor.org/packages... ▸ Link to code: https://github.com/kpatel427/YouTubeT... Chapters: 0:00 Intro 1:14 Main effect vs Interaction terms 3:09 Case 1: One factor with 2 groups 5:14 Case 2: One factor with multiple groups 6:34 Case 3: Two factors with 2 groups each 8:28 Does the order matter? [~ genotype + treatment] vs [~ treatment + genotype] 9:59 Understanding Interaction terms 11:58 When to use interaction terms? 18:01 Case 4: Two factors with multiple groups (without interaction terms) 19:47 Case 4: Two factors with multiple groups (with interaction terms) 20:42 Difference between [~ genotype:treatment] and [~ genotype + treatment + genotype:treatment] Show your support and encouragement by buying me a coffee: https://www.buymeacoffee.com/bioinfor... To get in touch: Website: https://bioinformagician.org/ Github: https://github.com/kpatel427 Email: [email protected] #bioinformagician #bioinformatics #deseq2 #deseq #edger #signac #atacseq #atac #seurat #singlecell #singler #illumina #bridgeamplification #sequencingbysynthesis #multiplex #alleles #10x #oxfordnanopore #pacbio #affymetrix #barcode #setseed #reproducibility #pseudorandom #singleR #singlecell #annotationdbi #reversestranded #directstranded #strandedness #survival #survminer #survivalanalysis #kaplanmeier #tcga #gdcportal #tcgaportal #nci #cran #bioconductor #funcotator #variantcalling #variants #gatk #vcf #gvcf #haplotype #alleles #geneticvariants #mutations #gff3 #gff #gtf #sam #bam #phred #fasta #fastq #singlecell #10X #ensembl #biomart #annotationdbi #annotables #affymetrix #microarray #affy #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #GEO #rnaseq #ngs