Скачать с ютуб Why is NGS Vital to the Clearing of Chronic UTI's? | MicroGenDX Minute Ep. 5 в хорошем качестве

Why is NGS Vital to the Clearing of Chronic UTI's? | MicroGenDX Minute Ep. 5

To learn how MicroGenDX can help your physician identify the microbes causing your chronic infection, visit microgendx.com Studies have shown that 80% of Chronic UTIs are caused by biofilm infections- communities of microbes living together- and not planktonic bacteria as previously believed. Research since the 70s has increasingly shown us that the tendency of bacteria is to develop into a biofilm state. Bacteria create biofilm formations on the surfaces inside the body, and biofilms become collaborative communities of different species of bacteria and fungi. It is possible for anaerobes, aerobes, and fungi to exist in the same biofilm. Its because of these special behaviors that a biofilm cannot be accurately identified with cultures. Its because of these special behaviors that a biofilm cannot be accurately identified with cultures. First, because the biofilm will not form in a culture dish the way it has inside the host body. Once the biofilm is broken up the species which make it up revert to their planktonic forms of behavior and those species which flourish in the environment of the dish grow even if they are only minor players in the biofilm community, and others may be unable to grow even if they were the majority of the biofilm in the host’s infection. Moreover, because the biofilm can be made of different kinds of bacteria and fungi, it would take multiple kinds of cultures to identify them all even if they could all be grown in the lab, which many species cannot. This explains why patients with UTI symptoms so often have culture tests come back negative- because biofilms simply cannot be grown in cultures. Molecular testing does not face these difficulties because it does not attempt to grow any of the microbes, but rather extracts the DNA from the sample. The two most commonly used tests for infectious disease are Polymerase Chain reaction- also known as PCR and a more advanced DNA test- Next generation DNA sequencing, known as, NGS. PCR compares the DNA in a sample against a panel of microbial species which are believed to be common pathogens in that area of infection. These panels are limited to a predetermined list of species which represent 1% or fewer of all known microbes. If you’re only using PCR, you may be missing out on important diagnostic information. MGDX two part DNA test, PCR and NGS, compares the DNA in a sample against our curated database of more than 50,000 microbial species to accurately identify all of the species in a sample. MicroGenDX’s two part DNA test can discover aerobic and anaerobic bacteria and fungi in a sample- all microbes identified in one test at one price, so that you can target all of the causative microbes at once. Also, our report provides information cultures simply can’t, to accurately identify all of the species in a sample. Furthermore, MicroGenDX’s report lists each species by their relative percentage of the total. This provides a complete microbial picture to assist doctors in determining which species need to be targeted in order to clear the infection. The MicroGen DX report identifies ten common antibiotic resistance genes- which would change the efficacy of arounds 40 kinds of antibiotics. A sensitivity report takes those species which can grow in the lab and tests their response to the presence of antibiotics. But all this test can tell you is if the bacteria in the dish are sensitive to a select antibiotic. This test can’t tell you if the bacteria has the genes for antibiotic resistance- after all, genes can be switched on or off. The gene maybe present and active in the host when its inactive in the dish. Or the gene for resistance may exist in a species which couldn’t grow in the dish. We know that bacteria can share genes for antibiotic resistance with other species in a biofilm infection. What you need to know is ALL of the microbial species in the sample, which species are dominant, and what antibiotic resistance genes are present. Having the complete picture allows you to provide targeted treatment, and the complete picture is only available through MicroGenDX To learn more, visit microgendx.com