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TEPTU 2016. Rumsha Javed

MIDWOOD HIGH SCHOOL DNA-damage Induced and p53-dependent Nucleolin Translocation in Breast Cancer Cells. Breast cancer is one of the most common cancers found in women living in the United States. There are two main categories of breast cancer: triple negative and estrogen/progesterone positive. There are also molecular subtypes of breast cancer such as luminal a, luminal b, or basal. In this experiment, two specific breast cancer cell lines, MCF7 and T47D, were focused upon. Both belong to the same breast cancer type and molecular subtype but their p53 status varies with MCF7 containing wild-type p53 and T47D containing mutant p53. Nucleolin, the protein that was of major concern, is a non-ribosomal phosphoprotein that is often found in the nucleolus, but can also be found in nucleus and cytoplasm. When reacting to DNA damage, nucleolin can localize out of its original place which can stop RPA functions in DNA replication. The relocalization depends on the p53, more specifically the C-terminus, and cellular stress. In this experiment, the nucleolin response to various cellular stress was observed by examining its localization in the mentioned cell lines. After observing the results, it was found that nucleolin localized to the nucleoplasm in the MCF7 cell line under UV cellular stress. Nucleolin stayed in the nucleolus in MCF7 under CPT and control conditions. On the other hand, the nucleolin in T47D stayed in the nucleolus even under cellular stress. In conclusion, T47D and MCF7 have showed different nucleolin localization patterns. This may largely be due to the p53 status.