Скачать с ютуб Mycology Lab 101: Agar Work, Cloning, Spores & Sterile Culture Technique for Mushroom Cultivation в хорошем качестве

Mycology Lab 101: Agar Work, Cloning, Spores & Sterile Culture Technique for Mushroom Cultivation

Master mycology lab skills with this deep-dive video about advanced sterile culture technique for mushroom cultivation! Learn everything you need to know about working with mycelium on petri dishes, making agar transfers, inoculating grain spawn and liquid culture, cloning live mushrooms and germinating and isolating new cultures from a spore print. Sterile culture technique allows us to breed new strains, preserve biological specimens and prevent contamination, opening new doors in mycology and mushroom cultivation. But it requires special attention and delicate precision, so having some solid guidance at the beginning of your journey will save you lots of headaches and trouble down the road. In part 1, we’ll cover all the important equipment, supplies, tools and concepts, including how to set up a low-cost, do-it-yourself laboratory, all of the essential principles of sterile culture technique, its value and its limitations. We’ll discuss contamination, how to use Parafilm, and some helpful tricks for tracking your workflow. In part 2, we’ll learn how to use a scalpel to make agar transfers and work with mushroom cultures on petri dishes. We’ll go into detail about agar to agar, agar to grain, agar to liquid culture, how to verify your work with a streak test and some tips for scaling up. Part 3 demonstrates how to get a mushroom culture started in the first place, starting with tips for sourcing new genetics. Then we explain how to clone a mushroom using sterile tissue culture, and finally how to germinate spores and isolate new mushroom strains on petri dishes. With just this handful of techniques, you can unlock the next level in your mushroom cultivation skill tree, and cast whatever kind of mushroom magic you desire! Made with love by Fungaia, a heart-centered, donation-based mushroom company https://www.fungaia.life/ For the best, most reliable and super affordable mushroom genetics and liquid culture, visit our favorite culture library: True Blue Genetics https://www.TrueBlueGenetics.org/?cou... Discount code: FUNGAIA ____________________________ Supplies List 1. Mushroom Genetics Gourmet and Medicinal Mushroom Cultures from Fungaia https://www.fungaia.life/shop/species/ The best liquid culture on the market: True Blue Genetics https://truebluegenetics.org/?coupon=... This is the last genetics provider you will ever need! 2. Implements Scalpel #7 Handle: https://amzn.to/3UNwW1C #11 Blades: https://amzn.to/4bDrNiK Inoculation Loop: https://amzn.to/3UQNRR0 Forceps: https://amzn.to/3wq1I6H 3. Essential Lab Supplies Alcohol Lamp: https://amzn.to/3TgSClX Nitrile Gloves: https://amzn.to/49imAvc Paper Towels Denatured Alcohol (for lamp) 70% Isopropyl Alcohol + Spray Bottle Pre-Poured Petri Dishes https://agarnow.com/collections/petri... Fungaia Laminar Flow Hood https://www.fungaia.life/shop/flowhood/ ____________________________ Other Useful Supplies The Fungaia Cube Kit https://www.fungaia.life/shop/kits/?q... An organic, all-in-one grow kit for dung-lover mushrooms Pre-Sterilized Grain Bags https://www.fungaia.life/shop/fertile... Pasteurized, Manure-Based Mushroom Substrate https://www.fungaia.life/shop/fertile... Monotubs and monotub filters: Microppose https://microppose.com/r?id=h776jn Discount code FUNGAIA Bulk Liquid Culture IV Bags https://www.fungaia.life/shop/spawn/?... ____________________________ More Videos from Fungaia How to Grow Mushrooms the Easy Way:    • How to Grow Mushrooms from Scratch th...   How to Make Grain Spawn:    • How to Make Grain Spawn: A Deep Dive ...   How to make your own liquid culture    • How to Make Liquid Culture the Easy W...   Advanced liquid culture method    • How to Make Liquid Culture: DIY Mushr...   How to make your own injection port jar lids:    • How to Make Injection Port Jar Lids f...   ____________________________ Upcoming videos: Introduction to Mushroom Cultivation How to Make Agar Media and Pour Petri Dishes The Basics of Breeding Storing Genetics and Managing a Mushroom Culture Library Troubleshooting Contamination: Identification, Prevention and Problem-Solving Created by Paul Lynn. All rights reserved. Made using some free stock footage. © 2024 Alembic Enterprises LLC Walla Walla, Washington Please forgive the video/edit quality, I’m still learning! 0:00 Intro 1:11 Overview 4:27 Background Info 8:58 Part 1: Equipment, Space & Supplies 17:27 Implements 20:53 How to Use Parafilm 24:42 Genetics 26:22 Part 2: Principles of SCT 26:37 Contamination 28:40 Degrees of Sterility 31:47 Priority of Sterility 33:18 Green, Yellow, Red Light 35:41 Goof and Grow 38:23 Part 2: The Juicy Stuff 41:48 Agar to Agar 45:53 Agar to Grain 48:49 Agar to Liquid Culture 50:03 Streak Test 50:44 Part 3: Starting a New Culture 51:06 Sourcing Genetics 52:01 Cloning a Mushroom 56:24 Spore Work 1:02:59 Outro + Love