Скачать с ютуб Exploring volume electron microscopy (vEM) and Transmission Electron Microscopy (TEM) в хорошем качестве

Exploring volume electron microscopy (vEM) and Transmission Electron Microscopy (TEM)

Volume Electron Microscopy (vEM) is back in the spotlight! Due in large part to Nature recognizing this powerful approach as one of its techniques to watch in 2023, interest in vEM has skyrocketed! Analogous to serial section TEM (ssTEM), vEM constructs large stacks of 2D EM images, which can be aligned and/or segmented to permit greater context to resin embedded cellular and tissue samples at very high resolution. vEM offers a large advantage over ssTEM through automation, FOV, and ease of use, without appreciable compromise. In this session, we will differentiate some of these approaches, compare/contrast the benefits of each, and present example data. Transmission electron microscopy (TEM) has been an important technology in cell and structural biology ever since it was first used in the early 1940s. The most frequently used TEM application in cell biology entails imaging-stained thin sections of plastic-embedded cells by the passage of an electron beam through the sample such that the beam will be absorbed and scattered, producing contrast and an image. Dr. de Val will present how the current TEM instruments at the Integrated Imaging Advanced Electron Microscopy Facility can be used for Life Sciences applications.