Скачать с ютуб Diagnosis of tuberculosis by whole genome sequencing of DNA from sputum - Kayzad Nilgiriwala в хорошем качестве

Diagnosis of tuberculosis by whole genome sequencing of DNA from sputum - Kayzad Nilgiriwala

Current screening of pulmonary tuberculosis (TB) via sputum microscopy only detects TB in 40-60% samples. Phenotypic drug susceptibility testing (DST) can take weeks or months. A faster test is available, but only predicts resistance to Rifampicin. Next-generation sequencing such as nanopore can detect TB and predict DST profiles ‘at a much faster rate’, demonstrating future potential to reduce time-to-diagnosis & appropriate treatment for TB. Sequencing DNA extracted directly from sputum performed on MinION, demonstrating detection of M. tuberculosis, lineage determination & DST profiling for 11 drugs, in samples with greater than 0.2% M. tb reads. ‘Nanopore sequencing can detect M. tb DNA directly from sputum of different grades (3+, 2+ and 1+)’. In future, Kayzad and team envision ‘nanopore sequencing as a point-of-care diagnostic tool’ for faster TB screening. Find out more at nanoporetech.com Follow us Twitter:   / nanopore   Facebook:   / oxford-nanopore-technologies-251034380246   LinkedIn:   / oxford-nanopore-technologies   Instagram:   / oxfordnanopore