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Presenter: Rachel Loo, Professor of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles In this tutorial lecture, presented on July 10, 2023 at the North American Mass Spectrometry Summer School, Rachel Loo shows how particle charging and ionization are indispensable steps in MS analysis. This lecture covers primary ways of achieving ionization and its impact on downstream analysis. To view the lecture schedule for past North American Mass Spectrometry Summer School events, visit https://www.ncqbcs.com/resources/trai.... Recorded videos for each lecture can be found on this YouTube channel. Website: https://www.ncqbcs.com/ The National Center for the Quantitative Biology of Complex Systems (NQBCS) was founded in 2016 through a grant from the National Institute of General Medical Sciences (GM108538) and is located on the campus of the University of Wisconsin-Madison. Our core mission is the development, application, and dissemination of mass spectrometry technology for proteomic analysis.