Скачать с ютуб 23. Prof. Andrew Ewing - Analytical Nanoelectrochemistry of Subcellular Organelles в хорошем качестве

23. Prof. Andrew Ewing - Analytical Nanoelectrochemistry of Subcellular Organelles

Full title: Analytical Nanoelectrochemistry of Subcellular Organelles Speaker: Prof. Andrew Ewing (University of Gothenburg, Sweden) First Part: 00:00 - Introduction 02:00 - Beginning of the talk 09:43 - Electroactive molecules in the brain/body 11:30 - Electrochemistry in living brains 27:45 - Quantitative probing of individual vesicles 34:42 - Intracellular Electrochemical Cytometry 40:00 - Mechanisms of VIEC First Q&A: 53:20 - Q1: Measurements of H2O2 inside the cell 53:46 - Q2: Electrical stimulation instead of chemical 57:42 - Q3: Combining fluorescence with electrochemistry 59:46 - Q4: Stimulation of neurons with nanoprobes 1:01:14 - Q5: The size of nanoprobes 1:03:12 - Q6: Electrochemical analysis of cells inside a TEM Second Part: 1:05:03 - Partial exocytotic release 1:14:35 - Drosophila as a model system 1:26:27 - Molecular dynamics simulations 1:23:30 - Exocytosis from beta cells 1:25:24 - Exocytosis from gut epithelium (BON) cells 1:26:41 - Nano-amperometry of intravesicular glutamate 1:31:46 - Dopamine storage probed by NanoSIMS 1:39:22 - Why is partial release important? 1:44:42 - Nanopipettes for local dosing of vesicles 1:49:10 - Dissecting nanometer vesicles 1:53:57 - Electrochemical analysis of stress granules 1:56:22 - Summary and conclusions Second Q&A: 1:57:53 - Q7: Electrode materials and grafted nanotips 2:00:06 - Q8: Other possible molecules to probe 2:00:53 - Q9: Electrode arrays instead of local probes 2:04:37 - Q10: Machine learning for exocytosis recognition 2:07:47 - Q11: Using nanoelectrodes for studying microbes 2:09:48 - Q12: Driving force for the vesicle adhesion to the surface 2:12:13 - Q13: Brain-computer interfaces 2:15:51 - Q14: In vivo monitoring of physiological parameters 2:17:31 - Q15: The future of the field https://www.electrochemicalcolloquium... #biochemistry #nano #neuroscience Abstract:  In recent years, micro-/nano-electrochemical approaches have been developed to realize the quantitative measurement of intravesicular content and real-time monitoring of vesicle release dynamics. Our group has developed new techniques based first on capillary electrophoresis followed by vesicle impact electrochemical cytometry (VIEC), and subsequently stochastic VIEC and intracellular VIEC (IVIEC) to provide a highly effective way to quantify the electroactive contents inside vesicles. We also use chemical imaging methods to study the vesicular systems. I will review the field of nanoelectrochemistry for intracellular analysis, emphasizing organelles and the analysis of their contents by direct and indirect (biosensing) methods. Following the more fundamental part, I will discuss recent results on analysis of analysis of the contents of intracellular vesicles, with an emphasis on neurotransmission and plasticity.